In Single-cell RNAseq analysis, there is a step to find the marker genes for
each cluster. The output from Seurat
FindAllMarkers
has a column called avg_log2FC
.
It is the gene expression log2 fold change between cluster x and all other clusters.
How is that calculated? In this tweet thread by Lior Pachter, he said that there was a discrepancy for the logFC changes between …